Composite

Part:BBa_K322999:Design

Designed by: Marta Bereska   Group: iGEM10_Edinburgh   (2010-10-08)


Blue light sensor (lovTAP) + Reporter system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 895
    Illegal SpeI site found at 903
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 895
    Illegal SpeI site found at 903
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 895
    Illegal SpeI site found at 903
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 895
    Illegal SpeI site found at 903
    Illegal AgeI site found at 1518
    Illegal AgeI site found at 1630
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 552


Design Notes

This blue light sensor is a fusion of a blue light sensitive protein with TrpR. Using a TrpR deletion strain might improve light sensitivity.


Source

Strickland, D., Moffat, K. & Sosnick, T. R. (2008). Light-activated DNA binding in a designed allosteric protein. Proceedings of the National Academy of Sciences 105, 10709-10714.

References